|
Products
Data Sets
Publications
Protocols
Images
Contribution Series
CAP LTER Symposia
|
|
Assessing Biodiversity of Arbuscular Mycorrhizal Fungi
Identifier:273_1
Publication date:2002
Author(s):
Jean Stutz
Jamaica Cousins
Robert Bills
Keywords:
Phoenix, Arizona, Sonoran Desert, caplter, Central Arizona Phoenix Longterm Ecological Research, urban, metropolitan area, mycorrhiza, Survey 200, caplter created, Project id 27, caplter core monitoring, Human Control of Biodiversity, Populations
Contact:
Data Manager,
Global Institute of Sustainability,Arizona State University,POB 875402,Tempe
caplter.data@asu.edu
Methods used in producing this dataset:
Field Protocol
collect x 3 soil samples using a hand trowel
for each sample dig to depth of 5 to 6 inches from a 4 x 4 inch square area
collect samples under 3 separate well-established shrubs and/or trees (adjacent to 9.5 m North, East and West of plot center if there is a lot of vegetation on the plot) or just under what is available if veg. is sparse (NB if no shrubs/trees present on plot, take outside up to distance of 50m from plot center)
choose representative species of shrub/tree if there is a variety of species
on plots with no veg. sample at 9.5 m North, East and West of plot center
put sample in Zip-Loc bag (should by 1/3 to ½ full) and label
- label bag WITH THE NAME OF THE SHRUB/TREE SPECIES FROM WHICH THE SAMPLE WAS COLLECTED UNDER
Lab Protocol
Store sample at 4 degrees C
Extract spores from a 50-100 cm3 subsample by wet sieving and sucrose density gradient centrifugation
Place washed spores into petri dish
Count all morphotypes. Use the Leica stereomicroscope
Mount a few spores of each morphotype on slides using polyvinyl alcohol-lactic acid-glycerol (PVLG) and PVLG mixed 1:1 (v/v) with Melzer’s reagent
Calculate the percentage of healthy appearing spores in each sample
Calculatespore density as the number of spores per 100 cm3 soil
Determine species richness as a count of the different AMF species detected at each site
Trap Cultures
Plant sudan grass (Sorghum sudanese (Piper) Staph.) seeds into 656 mL Deepots using a planting media consisting of a 1:1:1 mixture (175 cm3 each) of sample soil and steam-sterilized #20 and #12 grades silica sand and grown in a greenhouse.
Water every one to three days over the course of three months.
Upon flowering, stop watering, and allow plants to dry down.
Collect samples (100 cm3) from 25% of the first generation trap cultures and identify them using the same labritory protocol
For second generation traps use 175 cm3 of mixture from the first generation trap cultures.
Entities:
Table Name: 27_mycorrhiza_1.csv
download
Attributes:
Attribute:site_id
Description:Unique numbers and letters assigned to each site used in this study
Attribute:samp_date
Description:Encripted within the visit id
Attribute:landuse_label
Description:Unabbreviated landuse types
Attribute:level1
Description:First Catagory Tier
Attribute:parallel_id
Description:Parallel Identification; A, B, C
Attribute:trap_soil
Description:Species found in trap culture or original soil sample
Domain:
Enumeration:
soil: original soil sample
trap: trap sample
Attribute:mycoSpecies
Description:scientific species name
|
